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- 抗原
- Rpa12p (RPA12)
- 适用
- Arabidopsis thaliana
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- 预测反应
- Arabidopsis thaliana
- 产品特性
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Expected / apparent Molecular Weight of the Antigene: 13.6
This product has previously been labelled as anti-At3g25940 transcription factorS-II (TFIIS) domain-containing protein. - 纯化方法
- serum
- 免疫原
- KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
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- 应用备注
- 1 : 2 000 with standard ECL (WB)
- 说明
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This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 143 μL of sterile water
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- 抗原
- Rpa12p (RPA12)
- 别名
- A12.2
- 别名
- RRN4 antibody, DNA-directed RNA polymerase I subunit RPA12 antibody, RPA12 antibody
- 背景
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AGI Code: At3g25940
RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II. - 分子量
- 13.6
- UniProt
- Q9LU97
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