Rpa12p (RPA12) 抗体
The 兔 多克隆 anti-Rpa12p antibody is suitable to detect Rpa12p in samples from Arabidopsis thaliana. It has been validated for WB.
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产品编号 ABIN190724
发货至:
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Quick Overview for Rpa12p (RPA12) 抗体 (ABIN190724)
抗原
Rpa12p (RPA12)
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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原理
- Anti-A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Arabidopsis thaliana
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
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应用备注
- 1 : 2000 (WB)
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说明
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This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 143 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Rpa12p (RPA12)
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别名
- A12.2
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背景
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Background: RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.
Additional information: This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.Protocol for isolation of cytosolic and nuclear fractions can be found here.
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分子量
- 13.6 kDa
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基因ID
- 822191
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UniProt
- Q9LU97
抗原
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