Rubisco 抗体
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北京 101111
Quick Overview for Rubisco 抗体 (ABIN190723)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-Rubisco | 557 kDa hexadecamer
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交叉反应 （详细）
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Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Not reactive in: Chlamydomonas reinhardtii (immulolocalization) -
预测反应
- higher plants,algae,Anthoceros agrestis,Nannochloropsis sp.
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纯化方法
- Serum
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免疫原
- purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)
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anti-Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO) antibody
RuBisCO 适用: Arabidopsis thaliana WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO) antibodyRuBisCO 适用: 植物 WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO) antibodyRuBisCO 适用: 植物 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO) (Large Subunit) antibodyRuBisCO 适用: 植物 WB 宿主: 小鼠 Monoclonal 3G7 unconjugated
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应用备注
- 1 : 10 000-1 : 20 000 on 0,5-10 μg total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)
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说明
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RbcS subunit is not detected by this antibody
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).
: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." in: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).
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: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).
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- Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))
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别名
- Rubisco
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背景
- Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
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分子量
- 53-55 | 53-55 kDa
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基因ID
- 2715621
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UniProt
- P00875, Q43832
抗原
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