GLU I (PR-2) 抗体
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北京 101111
Quick Overview for GLU I (PR-2) 抗体 (ABIN190719)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-PR-2 | GLU I | Class I beta-1,3-glucanase
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交叉反应 （详细）
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Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Arabidopsis thaliana -
预测反应
- Dicots,Oryza sativa,Prunus persica
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免疫原
- Purified tobacco class I
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亚型
- IgG
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应用备注
- 8 μg/mL (WB)
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说明
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Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Total IgG in PBS pH 7.4. (without Ca++).
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Physiological compensation in antisense transformants: specific induction of an \" glucan endo-1,3-beta-glucosidase in plants infected with necrotizing viruses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 8792-6, (1993) (PubMed).
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- GLU I (PR-2)
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背景
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Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase
Additional information: For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51.This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.
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分子量
- 37 | 33 kDa
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基因ID
- 107801151
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UniProt
- P27666
抗原
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