GLU I (PR-2) 抗体
适用: Nicotiana tabacum, Potato
IL, WB
宿主: 兔
Polyclonal
unconjugated
1 image
(1 reference)-
- 抗原
- GLU I (PR-2)
- 适用
- Nicotiana tabacum, Potato
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunolocalization (IL), Western Blotting (WB)
- 交叉反应 （详细）
- No cross-reactivity with: Arabidopsis thaliana
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 37 / 33 kDa
- 纯化方法
- affinity purified
- 免疫原
- purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).
- 亚型
- IgG
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- 应用备注
- Recommended Dilution: 8 ug/mL with standard ECL (WB).
- 说明
-
Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL of sterile water.
- 缓冲液
- PBS pH 7.4 (without Ca++)
- 储存液
- Without preservative
- 注意事项
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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: "Physiological compensation in antisense transformants: specific induction of an \" glucan endo-1,3-beta-glucosidase in plants infected with necrotizing viruses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 8792-6, (1993) (PubMed).
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: "Physiological compensation in antisense transformants: specific induction of an \" glucan endo-1,3-beta-glucosidase in plants infected with necrotizing viruses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 8792-6, (1993) (PubMed).
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- 抗原
- GLU I (PR-2)
- 背景
- Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase
- 分子量
- expected: 37 kDa, apparent: 33 kDa
- UniProt
- P27666
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