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Topoisomerase I 抗体 (N-Term)

There are 2+ publications for this product available. The 小鼠单克隆 anti-Topoisomerase I antibody is suitable to detect Topoisomerase I in samples from 人. It has been validated for WB 和 FACS.

Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term) (ABIN1882055 and ABIN2838496). (Topoisomerase I 抗体 (N-Term))

Flow cytometric analysis of Hela cells using TOP1 Antibody (N-term)(green, Cat(ABIN1882055 and ABIN2838496)) compared to an isotype control of mouse IgG1(blue). (Topoisomerase I 抗体 (N-Term))

FACS

2 images

(2 references)

产品编号 ABIN1882055

发货至: 中国

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Quick Overview for Topoisomerase I 抗体 (N-Term) (ABIN1882055)

抗原

See all Topoisomerase I (TOP1) 抗体

Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Topoisomerase I antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS)

克隆位点

1291CT875-142-166

Discover our top product TOP1 Primary Antibody

抗原表位
11

7

5

3

3

2

2

2

1

1

1

1

1

1
N-Term

纯化方法

This antibody is purified through a protein G column, followed by dialysis against PBS.

免疫原

This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.

亚型

IgG1 kappa
anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibody
TOP1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated

anti-Topoisomerase (DNA) I (TOP1) antibody
TOP1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Topoisomerase (DNA) I (TOP1) antibody
TOP1 适用: 人 WB, ELISA, IHC, IP, FACS 宿主: 兔 Monoclonal 6D8 unconjugated Recombinant Antibody

anti-Topoisomerase (DNA) I (TOP1) antibody
TOP1 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) I (TOP1) (AA 1-100) antibody
TOP1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) I (TOP1) (Internal Region) antibody
TOP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Topoisomerase (DNA) I (TOP1) antibody
TOP1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB7195 unconjugated

anti-Topoisomerase (DNA) I (TOP1) antibody
TOP1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB2060 unconjugated Recombinant Antibody

anti-Topoisomerase (DNA) I (TOP1) antibody
TOP1 适用: 人, 大鼠 WB, FACS 宿主: 小鼠 Monoclonal 1C6 unconjugated

anti-Topoisomerase (DNA) I (TOP1) (AA 358-501) antibody
TOP1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. FC: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

有效期

6 months
Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

Kunze, Yang, Dölberg, Sundarp, Knippers, Richter: "Structure of the human type I DNA topoisomerase gene." in: The Journal of biological chemistry, Vol. 266, Issue 15, pp. 9610-6, (1991) (PubMed).
抗原

Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

别名

TOP1

背景

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

分子量

90726

UniProt

P11387

途径

Caspase Cascade in Apoptosis, Stem Cell Maintenance