Aggrecan 抗体
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北京 101111
Quick Overview for Aggrecan 抗体 (ABIN187628)
抗原
See all Aggrecan (ACAN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Reacts with chondroitin sulfate proteoglycan from cartilage (aggrecan). Species Reactivity: Reacts with human. No crossreactivity with bovine nasal cartilage, rat chondrosarcoma, chick or shark cartilage aggrecan. Other species not tested. Controls: Purified antigen, cartilage extracts or mature cultured chondrocytes.
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免疫原
- Human large molecular weight proteoglycan of articular cartilage (aggrecan).
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亚型
- IgG1
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anti-Aggrecan (ACAN) (AA 101-220) antibody
ACAN 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (AA 34-147) antibodyACAN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (AA 34-147) antibodyACAN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (AA 56-155) antibodyACAN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A8 unconjugated
anti-Aggrecan (ACAN) (AA 601-700) antibodyACAN 适用: 大鼠, 小鼠, 兔 ICC, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (N-Term) antibodyACAN 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (C-Term) antibodyACAN 适用: 人, 大鼠, Hamster, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (AA 587-684) antibodyACAN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (AA 2162-2316) antibodyACAN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aggrecan (ACAN) (AA 2163-2316) antibodyACAN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: 1:500-1:1,000 (highest ELISA reactivity of our 3 anti-chondroitin/keratan sufate proteoglycans). In solid phase immunoassays, endo-beta-galactosidase digestion of aggrecan increases the immunoreactivity of the antibody. Also reacts with papain-digested aggrecan. Immunohistochemistry: (paraffin embedded): 1:50-1:3,000. Treat tissue sections with 2 mg/ml testicular hyaluronidase in 50 mM Na-acetate buffer, pH 5.5 for 1 hr at 37C, followed by treatment with 0.2 mg/ml Protease XXIV in TBS, pH 7.2, for the same time and temperature. Immunoprecipitation: 1:50-1:300 Western blotting: 1:100-1:300 Reacts with 3 trypsin-generated fragments of human cartilage aggrecan (prior digested with chondroitinase and endo-beta-galactosidase) which have apparent MW of 55, 65 and 95 kDa. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 µl ascites containing 0.01% sodium azide. Antibody concentration >1 mg/ml.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Aggrecan (ACAN)
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别名
- Aggrecan
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途径
- Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
抗原
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