HSP70 抗体
Quick Overview for HSP70 抗体 (ABIN187606)
抗原
See all HSP70 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This clone recognizes both the constitutive (Hsp73) and inducible (Hsp72) forms of Hsp70. Species Reactivity: Reacts with Human, Monkey and Bovine. Other species not tested. Positive Control: BT474 or HeLa cells. Breast carcinoma.
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免疫原
- Hsp70 protein from HeLa cells.Produced by fusion between BALB/c spleno-cytes and mouse myeloma NS-1 cells.
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亚型
- IgG2a
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anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, ICC, FACS, IEM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated
anti-Heat Shock Protein 70 (HSP70) antibodyHSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 70 (HSP70) antibody (HRP)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal HRP
anti-Heat Shock Protein 70 (HSP70) antibody (FITC)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal FITC
anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Biotin
anti-Heat Shock Protein 70 (HSP70) antibody (PE)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PE
anti-Heat Shock Protein 70 (HSP70) antibody (APC)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal APC
anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PerCP
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 594)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 594
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 488
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应用备注
- Flow Cytometry Immunofluorescence (Not assessed) Immunoprecipitation: Use at 2 µg/mg protein lysate. Use Protein A. Western blotting: 0.5-1 µg/ml for 2 hrs. at RT Immunohistology: Formalin/paraffin - Use at 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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缓冲液
- Clone W27 is a monoclonal antibody against Heat Shock Factor 2. It is available at a concentration of 100ug/500ul in 10 mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Purified from ascites fluid by Protein A chromatography.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- HSP70 (Heat Shock Protein 70 (HSP70))
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别名
- Heat shock protein 70 / HSP70
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背景
- A wide variety of environmental and patho-physiological stress conditions trigger the synthesis of a family of proteins known as heat shock proteins, more appropriately called stress response proteins. Many heat shock proteins, including members of the Ssp70 family, are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation and secretion. Hsp70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other unknown proteins. Also, Hsp70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs and other damaging conditions.
抗原
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