PARP4 抗体 (AA 491-494)
Quick Overview for PARP4 抗体 (AA 491-494) (ABIN187330)
抗原
See all PARP4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 491-494
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特异性
- This clone (P193-4) reacts with an internal epitope (amino acids 491-494, HPGE) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.
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免疫原
- Clone p193-4 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
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亚型
- IgG2b
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anti-Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4) (AA 338-609) antibody
PARP4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4) antibodyPARP4 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4) (Internal Region) antibodyPARP4 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4) (AA 31-140) antibodyPARP4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4) (AA 1-300) antibodyPARP4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. Flow Cytometry: Optimal conditions have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- This antibody contains approximately 250ug of immunoglobulin per ml. The solution contains 1% BSA and 0.1% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))
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别名
- PARP4
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背景
- VPARP is an antibody to the minor vault protein.
抗原
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