Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
特异性
This clone (P193-10) reacts with an internal epitope (amino acids 506-510, VALGK) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.
免疫原
Clone p193-10 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.
PARP4
适用: 大鼠, Pig
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 750
应用备注
Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.
限制
仅限研究用
状态
Liquid
缓冲液
This antibody contains approximately 250ug of immunoglobulin per ml. The solution contains 1% BSA and 0.1% sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
抗原
PARP4
(Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))