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ADAM8 抗体 (AA 145-493)

Name: ADAM8 抗体 (AA 145-493)
SKU: ABIN1866534
Availability: OutOfStock
Rating: 5 (1 reviews)

ADAM8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 145-493) antibody (ABIN1866534)

Western Blotting (WB) image for anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 145-493) antibody (ABIN1866534)

Used in DAB staining on fromalin fixed paraffin-embedded Liver tissue

IHC

4 images

(1 validation)

产品编号 ABIN1866534

发货至: 中国

Datasheet as PDF

抗原 See all ADAM8 抗体

ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))
抗原表位
16

10

9

6

5

5

2

2

1

1

1

1

1

1

1
AA 145-493
适用
60

23

6

4

4

1

1
小鼠
宿主
50

7

3
兔
Compatible Secondaries
克隆类型
52

8
多克隆
标记
25

5

5

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ADAM8 antibody is un-conjugated
应用范围
35

26

21

8

8

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

原理

Polyclonal Antibody to A Disintegrin And Metalloprotease 8 (ADAM8)

特异性

The antibody is a rabbit polyclonal antibody raised against ADAM8. It has been selected for its ability to recognize ADAM8 in immunohistochemical staining and western blotting.

交叉反应

人, 大鼠

纯化方法

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

免疫原

Recombinant A Disintegrin And Metalloprotease 8 (ADAM8) corresdonding to Glu145~Cys493 with N-terminal His Tag

亚型

IgG

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anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 677-824) antibody
ADAM8 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 763-824) antibody
ADAM8 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 51-150) antibody
ADAM8 适用: 人, 小鼠 ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (Internal Region) antibody
ADAM8 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 70-119) antibody
ADAM8 适用: 人, 小鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 371-587) antibody
ADAM8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (C-Term) antibody
Verified ADAM8 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 28-111) antibody
ADAM8 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-ADAM Metallopeptidase Domain 8 (ADAM8) (AA 17-300) antibody
ADAM8 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
生效 #104303 (Western Blotting)

by

Department of Neurosurgery, Philipps-University Marburg

No.

#104303

日期

2021.02.11

抗原

ADAM8

Lot Number

PAA620Mu01

Method validated

Western Blotting

Positive Control

Wildtype bone marrow derived mouse macrophages

Negative Control

Adam8 knock-out bone marrow derived mouse macrophages

Notes

Passed. ABIN1866534 specifically recognizes Adam8 in lysates of bone marrow derived mouse macrophages.

Validation Images

Western blot analysis of cell lysates from wildtype (WT) or Adam8 knock-out (KO) bone marrow derived mouse macrophages with ABIN1866534 (upper panels) or a beta-tubulin loading control (lower panels). Primary antibody incubation in TBST containing 5% milk (left) or 5% BSA (right).
Full Methods
Primary Antibody

ABIN1866534

Secondary Antibody

ABIN269949

Full Protocol

Isolate and subsequently grow Adam8 wt or ko bone marrow derived mouse macrophages in DMEM (Gibco, 11965-092, lot 2258599) supplemented with FBS superior (Sigma Aldrich, S0615, lot 0001640840), Pen Strep (Gibco, 15140-122, lot 2240835), and Murine M-CSF (PeproTech, 315-02, lot 0419245) at 37°C and 5% CO₂ in 2 mL on a 6 well plate to 70% confluency.
Lyse 800,000 cells in 50 µL RIPA buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% (v/v) NP-40, 0.5% (w/v) sodium deoxycholate, 0.1% (w/v) SDS, 10 mM phenanthrolin, 10 mM EDTA, phosphatase and proteinase inhibitor).
Determine total protein content of the lysates using Pierce BCA Protein Assay Kit (Thermo Scientific, 23225, lot RH237553).
Denature 10 µg of total protein for 5 min at 95 °C in 1x Laemmli Buffer and subsequently separate them on a denaturing 10% SDS PAGE gel (polyacrylamide, Tris-HCl pH 6.8 for the stacking gel and pH 8.8 for separating gel, water, ammonium persulfate, SDS, tetramethyldiamine in a Mini-PROTEAN Tetra System (BioRad) for 1.5 h at 120 V.
Transfer proteins onto nitrocellulose membrane (GE Healthcare Life Science, 10600001, lot A29635973) with a Western blotting system for 70 min at 150 mA.
Block the membrane with TBST containing 5% milk or 5% BSA for 1 h at RT.
Incubation with primary
rabbit anti-ADAM8 antibody (antibodies-online, ABIN1866534, PAA620Mu01) diluted 1:1,000 in TBST containing 5% milk or 5% BSA for over night at 4°C.
rabbit-anti TUBB antibody (Novus Biologicals, NB600-936, lot J) diluted 1:2,000 in TBST containing 5% milk over night at 4°C.
Wash membrane 3x for 10 min with TBST.
Incubation with secondary Donkey Anti-Rabbit IgG H&L (HRP) (abcam, ab97064, lot GR3209122-1) diluted 1:5,000 in TBST containing 5% milk for 1h at RT.
Wash membrane 3x for 10 min with TBST.
Reveal protein bands using Western Bright Chemilumineszenz Substrat Sirius (Biozym, 541021, no 200331-75) on a Developing machine, Royal Intas ChemoCam Imager (Inta Science Imaging) exposure time 20 sec (TBST with 5% milk) or 3 sec (TBST with 5% BSA).

Experimental Notes

ABIN1866534 reveals protein bands and 90 kDa and 110 kDa specifically in the Adam8 wildtype lysates. They are not present in the Adam8 ko lysates. And additional unspecific band between these two bands in visible in all lysates.
Blocking with TBST containing 5% milk gives clearer results that blocking with TBST containing 5% BSA.
状态

Liquid

浓度

Lot specific

缓冲液

0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

储存液

ProClin

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
抗原

ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

别名

A Disintegrin And Metalloprotease 8 (ADAM8 产品)

别名

CD156 antibody, MS2 antibody, CD156a antibody, E430039A18Rik antibody, adam8 antibody, reprolysin antibody, si:zc217g15.7 antibody, zgc:64059 antibody, ADAM8 antibody, RGD1566394 antibody, ADAM metallopeptidase domain 8 antibody, a disintegrin and metalloproteinase domain 8 antibody, a disintegrin and metallopeptidase domain 8 antibody, ADAM metallopeptidase domain 8a antibody, ADAM8 antibody, LOC100304737 antibody, Adam8 antibody, adam8a antibody, adam8 antibody

背景

CD156-A, MS2, CD156a, Disintegrin and metalloproteinase domain-containing protein 8

途径

Activation of Innate immune Response, M Phase