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alpha Fetoprotein 抗体 (AA 31-171) (FITC)

This anti-alpha Fetoprotein antibody is a 兔 多克隆 antibody detecting alpha Fetoprotein in WB, IHC, IF 和 ICC. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN1857933
发货至: 中国

Quick Overview for alpha Fetoprotein 抗体 (AA 31-171) (FITC) (ABIN1857933)

抗原

See all alpha Fetoprotein (AFP) 抗体
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

适用

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宿主

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克隆类型

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多克隆

标记

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This alpha Fetoprotein antibody is conjugated to FITC

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 31-171

    原理

    FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)

    序列

    MGHHHHHHSG SEF- ILDSYQCTAE ISLADLATIF FAQFVQEATY KEVSKMVKDA LTAIEKPTGD EQSSGCLENQ LPAFLEELCH EKEILEKYGH SDCCSQSEEG RHNCFLAHKK PTPASIPLFQ VPEPVTSCEA YEEDRETFMN KFIYEIARRH P

    特异性

    The antibody is a rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in immunohistochemical staining and western blotting.

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    Recombinant aFP expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against aFP conjugated to fitc.

    亚型

    IgG
  • 应用备注

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    500 μg/mL

    缓冲液

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    注意事项

    Avoid repeated freeze/thaw cycles

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • Yang, Chen, Wang, Xia, Cheng, Pei, Wang, Bao: "Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip." in: Biosensors & bioelectronics, Vol. 48, pp. 153-7, (2013) (PubMed).

  • 抗原

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    别名

    Alpha-Fetoprotein

    背景

    AFP, A-FP, FETA, HPAFP, Alpha-Fetoglobulin, Alpha-1-fetoprotein

    途径

    C21-Steroid Hormone Metabolic Process
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