Leptin Receptor 抗体 (Internal Region)
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Quick Overview for Leptin Receptor 抗体 (Internal Region) (ABIN185639)
抗原
See all Leptin Receptor (LEPR) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Leptin Receptor
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序列
- TQDDIEKHQS DAG
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特异性
- This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).
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交叉反应
- 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2, NP_001003679.1, NP_001003680.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.
Western Blot: Approx 140-150 kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132 kDa according to Human NP_002294.2 and 130.8 kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Leptin Receptor (LEPR)
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别名
- LEPR
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背景
- LEPR , leptin receptor , CD295, OBR , OB receptor, LeprB
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基因ID
- 3953
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NCBI登录号
- NP_002294, NP_001003679, NP_001003680
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途径
- JAK/STAT Signaling, AMPK Signaling, Feeding Behaviour
抗原
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