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Choline Acetyltransferase 抗体 (C-Term)

This 山羊 多克隆 anti-Choline Acetyltransferase antibody specifically detects Choline Acetyltransferase in WB, IHC 和 ELISA. The antibody is reactive with 小鼠 和 大鼠 samples.
产品编号 ABIN185635
发货至: 中国
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Quick Overview for Choline Acetyltransferase 抗体 (C-Term) (ABIN185635)

抗原

See all Choline Acetyltransferase (CHAT) 抗体
Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

适用

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小鼠, 大鼠

宿主

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山羊

克隆类型

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  • 28
多克隆

标记

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This Choline Acetyltransferase antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    Chat (mouse)

    序列

    KERARGPTKP SNLD

    特异性

    This antibody is NOT expected to cross-react to Human CHAT.

    交叉反应

    小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.

    Western Blot: Approx 75 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3 kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

    别名

    Chat

    背景

    Chat, choline acetyltransferase , B230380D24Rik

    基因ID

    12647, 432359

    NCBI登录号

    NP_034021

    途径

    Skeletal Muscle Fiber Development
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