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Amphiphysin 抗体 (Internal Region)

This anti-Amphiphysin antibody is a 山羊 多克隆 antibody detecting Amphiphysin in WB, ELISA 和 IHC. Suitable for 人 和 小鼠.
产品编号 ABIN185628
发货至: 中国

Quick Overview for Amphiphysin 抗体 (Internal Region) (ABIN185628)

抗原

See all Amphiphysin (AMPH) 抗体
Amphiphysin (AMPH)

适用

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人, 小鼠

宿主

  • 63
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  • 1
山羊

克隆类型

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多克隆

标记

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This Amphiphysin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Amphiphysin / AMPH

    序列

    KRKDESRISK AE

    特异性

    This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1, NP_647477.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.

    Western Blot: Approx 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997

    Peptide ELISA: antibody detection limit dilution 1:16000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Amphiphysin (AMPH)

    别名

    AMPH

    背景

    AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen) , HGNC:471, AMPH1

    基因ID

    273, 218038

    NCBI登录号

    NP_001626, NP_647477
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