Iba1 抗体 (Internal Region)
Quick Overview for Iba1 抗体 (Internal Region) (ABIN185597)
抗原
See all Iba1 (IBA1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- AIF1 / IBA1 (isoform 3)
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序列
- NKQFLDDPKY SSDED
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特异性
- This antibody is expected to recognise isoform 3 (NP_001614.3) only.
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交叉反应
- 犬, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of K562 cells
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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别名
- AIF1
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背景
- AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
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基因ID
- 199, 11629
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NCBI登录号
- NP_001614
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途径
- Smooth Muscle Cell Migration
抗原
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