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Iba1 抗体 (Internal Region)

The 山羊 多克隆 anti-Iba1 antibody is suitable to detect Iba1 in samples from 人. It has been validated for IHC, ELISA, IF 和 FACS.
产品编号 ABIN185597
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Iba1 抗体 (Internal Region) (ABIN185597)

抗原

See all Iba1 (IBA1) 抗体
Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Iba1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    AIF1 / IBA1 (isoform 3)

    序列

    NKQFLDDPKY SSDED

    特异性

    This antibody is expected to recognise isoform 3 (NP_001614.3) only.

    交叉反应

    犬, 人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of K562 cells

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

    别名

    AIF1

    背景

    AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

    基因ID

    199, 11629

    NCBI登录号

    NP_001614

    途径

    Smooth Muscle Cell Migration
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