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EN2 抗体 (C-Term)

The 山羊 多克隆 anti-EN2 antibody is suitable to detect EN2 in samples from 人 和 小鼠. It has been validated for WB, ELISA 和 IF.
产品编号 ABIN185576
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EN2 抗体 (C-Term) (ABIN185576)

抗原

See all EN2 抗体
EN2 (Engrailed Homeobox 2 (EN2))

适用

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人, 小鼠

宿主

  • 53
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山羊

克隆类型

  • 53
  • 3
多克隆

标记

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This EN2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 18
    • 15
    • 8
    • 5
    • 1
    C-Term

    原理

    Engrailed-2

    序列

    NHSTTAKEGK SDSE

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 35 kDa band observed in Mouse Brain lysates (calculated MW of f 33.8 kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    EN2 (Engrailed Homeobox 2 (EN2))

    别名

    EN2

    背景

    Engrailed-2, engrailed homolog 2 , EN2, AUTS1

    基因ID

    2020

    NCBI登录号

    NP_001418
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