EN2 抗体 (C-Term)
Quick Overview for EN2 抗体 (C-Term) (ABIN185576)
抗原
See all EN2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Engrailed-2
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序列
- NHSTTAKEGK SDSE
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
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亚型
- IgG
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anti-Engrailed Homeobox 2 (EN2) (C-Term) antibody
EN2 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Engrailed Homeobox 2 (EN2) (AA 1-251) antibodyEN2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Engrailed Homeobox 2 (EN2) (AA 86-210) antibodyEN2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E1 unconjugated
anti-Engrailed Homeobox 2 (EN2) (C-Term) antibodyEN2 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Engrailed Homeobox 2 (EN2) (AA 243-271), (C-Term) antibodyEN2 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18338 unconjugated
anti-Engrailed Homeobox 2 (EN2) (AA 243-271), (C-Term) antibodyEN2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18334 unconjugated
anti-Engrailed Homeobox 2 (EN2) (AA 243-271), (C-Term) antibodyEN2 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB18198 unconjugated
anti-Engrailed Homeobox 2 (EN2) antibodyEN2 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Engrailed Homeobox 2 (EN2) antibodyEN2 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 35 kDa band observed in Mouse Brain lysates (calculated MW of f 33.8 kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:4000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- EN2 (Engrailed Homeobox 2 (EN2))
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别名
- EN2
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背景
- Engrailed-2, engrailed homolog 2 , EN2, AUTS1
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基因ID
- 2020
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NCBI登录号
- NP_001418
抗原
-
