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ERG 抗体 (C-Term)

This 山羊 多克隆 anti-ERG antibody specifically detects ERG in WB, ELISA, IF 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN185550
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for ERG 抗体 (C-Term) (ABIN185550)

抗原

See all ERG 抗体
ERG (ERG, ETS transcription factor (ERG))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This ERG antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    ERG

    序列

    PNTRLPTSHM PSH

    特异性

    This antibody is expected to recognise all reported isoforms (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1)

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 55 kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8 kDa according to NP_891548.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    ERG (ERG, ETS transcription factor (ERG))

    别名

    ERG

    背景

    ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets er

    基因ID

    2078, 13876, 170909

    NCBI登录号

    NP_891548, NP_004440, NP_001129626, NP_001129627
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