Tryptophan Hydroxylase 2 抗体 (Internal Region)
Quick Overview for Tryptophan Hydroxylase 2 抗体 (Internal Region) (ABIN185533)
抗原
See all Tryptophan Hydroxylase 2 (TPH2) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Tryptophan hydroxylase 2 / TPH2
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序列
- NKPNSGKNDD KGNK
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交叉反应
- Cow, 犬, 人, Pig
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-NKPNSGKNDDKGNK, from the internal region of the protein sequence according to NP_775489.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Brain Stem shows staining of the cytoplasm of Purkinje cells and of neuron projections. Recommended concentration, 2-4 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Tryptophan Hydroxylase 2 (TPH2)
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别名
- TPH2
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背景
- TPH2, tryptophan hydroxylase 2, HGNC:20692, FLJ37295, NTPH , neuronal tryptophan hydroxylase, ADHD7, MGC138871, MGC138872, tryptophan 5-monooxygenase 2
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基因ID
- 121278
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NCBI登录号
- NP_775489
抗原
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