CBX3 抗体 (Internal Region)
(1 reference)Quick Overview for CBX3 抗体 (Internal Region) (ABIN185493)
抗原
See all CBX3 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- CBX3 / HP1 Gamma
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序列
- EAFLNSQKAG KEKD
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特异性
- No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2, NP_057671.2.
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亚型
- IgG
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anti-Chromobox Homolog 3 (CBX3) (AA 1-183) antibody
CBX3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) (AA 1-183) antibodyCBX3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) (AA 1-183) antibodyCBX3 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6D1C4 unconjugated
anti-Chromobox Homolog 3 (CBX3) (AA 1-183) antibodyCBX3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G12-1D9 unconjugated
anti-Chromobox Homolog 3 (CBX3) (AA 1-183) antibodyCBX3 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) (AA 2-183) antibodyCBX3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) antibodyCBX3 适用: 人, 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) antibodyCBX3 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) (Internal Region) antibodyCBX3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 3 (CBX3) (Middle Region) antibodyCBX3 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration: 2-3 μg/mL.
Western Blot: Approx. 20 kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8 kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).
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: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).
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- CBX3 (Chromobox Homolog 3 (CBX3))
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别名
- CBX3
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背景
- CBX3, HP1-GAMMA, chromobox homolog 3 (HP1 gamma homolog, Drosophila) , HGNC:1553, HECH, HP1Hs-gamma , HP1 gamma homolog, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), heterochromatin protein HP1 gamma, heterochromatin-like protein 1
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基因ID
- 11335, 12417, 297093
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NCBI登录号
- NP_009207
抗原
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