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CBX5 抗体 (Internal Region)

This 山羊 多克隆 anti-CBX5 antibody specifically detects CBX5 in WB, ELISA, IF 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN185491
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CBX5 抗体 (Internal Region) (ABIN185491)

抗原

See all CBX5 抗体
CBX5 (Chromobox Homolog 5 (CBX5))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This CBX5 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    CBX5 / HP1-Alpha

    序列

    NKRKSNFSNS ADDIK

    特异性

    Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein.

    交叉反应

    犬, 人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1, NP_001120793.1, NP_001120794.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 24-25 kDa band observed in lysates of cell lines A43N and K562 (calculated MW of 22.2 kDa according to Human NP_036249.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    CBX5 (Chromobox Homolog 5 (CBX5))

    别名

    CBX5

    背景

    CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila), HGNC:1555, HP1, HP1Hs-alpha , chromobox homolog 5 (Drosophila HP1 alpha), Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein

    基因ID

    23468

    NCBI登录号

    NP_036249, NP_001120793, NP_001120794
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