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PEPCK 抗体 (Internal Region)

Name: PEPCK 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN185483
Availability: OutOfStock

This anti-PEPCK antibody is a 山羊多克隆 antibody detecting PEPCK in WB, ELISA, IF 和 FACS. Suitable for 人和小鼠. This Primary Antibody has been cited in 1 publication.

ABIN185483 (2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). (PEPCK 抗体 (Internal Region))

(ABIN185483) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (PEPCK 抗体 (Internal Region))

ABIN185483 (0. (PEPCK 抗体 (Internal Region))

(ABIN185483) (2 μg/mL) staining of A431 (A), HEK293 (B) and (1 μg/mL) HepG2 (C) cell lysate (35 μg protein in RIPA buffer). (PEPCK 抗体 (Internal Region))

.(ABIN185483) (2 μg/mL) staining of NIH3T3 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence. (PEPCK 抗体 (Internal Region))

(ABIN185483) Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0. (PEPCK 抗体 (Internal Region))

FACS

6 images

(1 reference)

产品编号 ABIN185483

发货至: 中国

Quick Overview for PEPCK 抗体 (Internal Region) (ABIN185483)

抗原

See all PEPCK 抗体

PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

适用

人, 小鼠

宿主

山羊

克隆类型

多克隆

标记

This PEPCK antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified

抗原表位
15

8

7

7

4

2

1

1

1

1

1
Internal Region

原理

PCK2 / PEPCK-M

序列

KPWKPGDKEP CAH

特异性

No cross-reactivity with PCK1.

交叉反应

Cow, 犬, 人, 小鼠, Pig, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2.

亚型

IgG
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibody
PEPCK 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 44-175) antibody
PEPCK 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3D3D9 unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibody
PEPCK 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 33-198) antibody
PEPCK 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibody
PEPCK 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibody
PEPCK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C9 unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-280) antibody
PEPCK 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 24-54), (N-Term) antibody
PEPCK 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3640 unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibody
PEPCK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 365-470) antibody
PEPCK 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7 kDa according to Human NP_004554.3 and 70.5 kDa according to Mouse NP_083270.2). Recommended concentration: 1-3 μg/mL. Primary incubatio
Peptide ELISA: antibody detection limit dilution 1:4000.

说明

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).
抗原

PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

别名

PCK2

背景

PCK2, PEPCK-M, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK2 , PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase

基因ID

5106

NCBI登录号

NP_004554

途径

Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis