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Caveolin-1 抗体 (Internal Region)

This anti-Caveolin-1 antibody is a 山羊 多克隆 antibody detecting Caveolin-1 in WB, IHC, ELISA, IF 和 FACS. Suitable for 人.
产品编号 ABIN185421
发货至: 中国

Quick Overview for Caveolin-1 抗体 (Internal Region) (ABIN185421)

抗原

See all Caveolin-1 (CAV1) 抗体
Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

适用

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宿主

  • 113
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山羊

克隆类型

  • 105
  • 32
多克隆

标记

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This Caveolin-1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Caveolin 1

    序列

    DELSEKQVYD AH

    特异性

    This antibody is expected to recognize both reported isoforms (NP_001744.2, NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

    交叉反应

    犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 22 kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5 kDa according to NP_001744.2). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at room temperature. Pre

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analy

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

    别名

    CAV1

    背景

    CAV1, caveolin 1, caveolae protein, 22 kDa , HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP08

    基因ID

    857, 12389, 25404

    NCBI登录号

    NP_001744, NP_001166366

    途径

    Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals
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