Caveolin-1 抗体 (Internal Region)
Quick Overview for Caveolin-1 抗体 (Internal Region) (ABIN185421)
抗原
See all Caveolin-1 (CAV1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Caveolin 1
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序列
- DELSEKQVYD AH
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特异性
- This antibody is expected to recognize both reported isoforms (NP_001744.2, NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3
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交叉反应
- 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 22 kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5 kDa according to NP_001744.2). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at room temperature. Pre
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analy
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))
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别名
- CAV1
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背景
- CAV1, caveolin 1, caveolae protein, 22 kDa , HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP08
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基因ID
- 857, 12389, 25404
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NCBI登录号
- NP_001744, NP_001166366
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途径
- Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals
抗原
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