AGRP 抗体 (C-Term)
Quick Overview for AGRP 抗体 (C-Term) (ABIN185385)
抗原
See all AGRP 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- AGRP / Agouti-related protein
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序列
- CRKLGTAMNP CSRT
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特异性
- This antibody is expected to recognise both reported isoforms (as represented by NP_001129, NP_015531)
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交叉反应
- 犬, 人, Pig
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CRKLGTAMNPCSRT, from the C Terminus of the protein sequence according to NP_001129.1, NP_015531.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration: 0.05-0.1 μg/mL.
Western Blot: Preliminary experiments gave bands at approx 45 kDa and 24 kDa in Human Brain lysates after 0.1 μg/mL antibody staining. Thiese bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temper
Peptide ELISA: antibody detection limit dilution 1:32000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
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别名
- AGRP
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背景
- AGRP, ART, AGRT, ASIP2, agouti related protein homolog (mouse), agouti (mouse) related protein, Agouti-related transcript, mouse, homolog of, MGC118963, agouti related protein homolog
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基因ID
- 181
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NCBI登录号
- NP_001129, NP_015531
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途径
- Feeding Behaviour, Photoperiodism
抗原
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