Acetylcholinesterase 抗体 (C-Term)
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Quick Overview for Acetylcholinesterase 抗体 (C-Term) (ABIN185357)
抗原
See all Acetylcholinesterase (AChE) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- ACHE
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序列
- QFDHYSKQDR CSDL
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特异性
- This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.
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亚型
- IgG
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anti-Acetylcholinesterase (AChE) (AA 398-617) antibody
AChE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (C-Term) antibodyAChE 适用: 人, 小鼠, 大鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 157-332) antibodyAChE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 147-175), (N-Term) antibodyAChE 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB16792 unconjugated
anti-Acetylcholinesterase (AChE) (AA 521-614) antibodyAChE 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 53-162) antibodyAChE 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) antibodyAChE 适用: 人 IHC, ELISA, IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 366-554) antibodyAChE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 147-175) antibodyAChE 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) antibodyAChE 适用: 人 WB, IHC, IF/ICC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Acetylcholinesterase (AChE)
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别名
- ACHE
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背景
- ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
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基因ID
- 43
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NCBI登录号
- NP_000656, NP_001269378
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途径
- Skeletal Muscle Fiber Development
抗原
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