Cytochrome B Reductase 1 抗体 (C-Term)
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Quick Overview for Cytochrome B Reductase 1 抗体 (C-Term) (ABIN185343)
抗原
See all Cytochrome B Reductase 1 (CYBRD1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Cytochrome b reductase 1
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序列
- CRNLALDEAG QRSTM
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特异性
- This antibody is expected to recognise isoform 1 (NP_079119.3) only.
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交叉反应
- Cow, 犬, 人, Pig
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3.
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亚型
- IgG
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anti-Cytochrome B Reductase 1 (CYBRD1) (AA 51-150) antibody
CYBRD1 适用: 人 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B Reductase 1 (CYBRD1) (AA 215-286) antibodyCYBRD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B Reductase 1 (CYBRD1) (AA 220-286) antibodyCYBRD1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B Reductase 1 (CYBRD1) antibodyCYBRD1 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B Reductase 1 (CYBRD1) (C-Term) antibodyCYBRD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Small Intestine. Rec
Western Blot: Preliminary experiments gave bands at approx 35 kDa and a 26+28 kDa doublet in human colon lysate after 0.1 μg/mL antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfull
Peptide ELISA: antibody detection limit dilution 1:16000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease." in: Journal of cellular and molecular medicine, Vol. 18, Issue 9, pp. 1840-50, (2014) (PubMed).
: "Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1816-26, (2012) (PubMed).
: "Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess." in: Molecular and cellular biochemistry, Vol. 321, Issue 1-2, pp. 123-33, (2009) (PubMed).
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: "Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease." in: Journal of cellular and molecular medicine, Vol. 18, Issue 9, pp. 1840-50, (2014) (PubMed).
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- Cytochrome B Reductase 1 (CYBRD1)
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别名
- CYBRD1
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背景
- CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3
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基因ID
- 79901
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NCBI登录号
- NP_079119
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途径
- Transition Metal Ion Homeostasis
抗原
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