Retinoid X Receptor alpha 抗体 (AA 14-28)
Quick Overview for Retinoid X Receptor alpha 抗体 (AA 14-28) (ABIN185295)
抗原
See all Retinoid X Receptor alpha (RXRA) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 14-28
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原理
- RXR alpha
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序列
- CQVNSSLTSP TGRGSM
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特异性
- This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CQVNSSLTSPTGRGSM, from the internal region of the protein sequence according to NP_002948.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))
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别名
- RXRA
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背景
- RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,
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基因ID
- 6256
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NCBI登录号
- NP_002948
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途径
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C
抗原
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