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Retinoid X Receptor alpha 抗体 (AA 14-28)

This anti-Retinoid X Receptor alpha antibody is a 山羊 多克隆 antibody detecting Retinoid X Receptor alpha in WB, ELISA, IF 和 FACS. Suitable for 人.
产品编号 ABIN185295
发货至: 中国

Quick Overview for Retinoid X Receptor alpha 抗体 (AA 14-28) (ABIN185295)

抗原

See all Retinoid X Receptor alpha (RXRA) 抗体
Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Retinoid X Receptor alpha antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    AA 14-28

    原理

    RXR alpha

    序列

    CQVNSSLTSP TGRGSM

    特异性

    This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence CQVNSSLTSPTGRGSM, from the internal region of the protein sequence according to NP_002948.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

    别名

    RXRA

    背景

    RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,

    基因ID

    6256

    NCBI登录号

    NP_002948

    途径

    Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C
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