EHD2 抗体 (C-Term)
Quick Overview for EHD2 抗体 (C-Term) (ABIN185287)
抗原
See all EHD2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- EHD2
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序列
- CRLVPPSKRR HKGSA
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特异性
- This antibody is expected to recognise EHD1 protein as well as EHD2.
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.
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亚型
- IgG
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应用备注
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Western Blot: Approx 65-70 kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2 kDa according to NP_055416.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. R
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- EHD2 (EH-Domain Containing 2 (EHD2))
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别名
- EHD2
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背景
- EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617
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分子量
- 61.2kDa
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基因ID
- 30846
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NCBI登录号
- NP_055416
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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