TCP1 alpha/CCTA 抗体 (C-Term)
Quick Overview for TCP1 alpha/CCTA 抗体 (C-Term) (ABIN185185)
抗原
See all TCP1 alpha/CCTA (TCP1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- TCP1
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序列
- SYEDAVHSGA LND
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特异性
- This antibody is expected to recognise both reported isoforms.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2 μg/mL.
Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3 kDa according to NP_110379.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
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别名
- TCP1
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背景
- TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1
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基因ID
- 6950
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NCBI登录号
- NP_110379, NP_001008897
抗原
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