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TCP1 alpha/CCTA 抗体 (C-Term)

This anti-TCP1 alpha/CCTA antibody (ABIN185185) is a Goat Polyclonal antibody detecting TCP1 alpha/CCTA in WB, ELISA, IHC, IF, FACS. Suitable for Human.
产品编号 ABIN185185
发货至: 中国

Quick Overview for TCP1 alpha/CCTA 抗体 (C-Term) (ABIN185185)

抗原

See all TCP1 alpha/CCTA (TCP1) 抗体
TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This TCP1 alpha/CCTA antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    TCP1

    序列

    SYEDAVHSGA LND

    特异性

    This antibody is expected to recognise both reported isoforms.

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2 μg/mL.

    Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3 kDa according to NP_110379.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

    别名

    TCP1

    背景

    TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1

    基因ID

    6950

    NCBI登录号

    NP_110379, NP_001008897
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