SH3BP1 抗体 (C-Term)
(1 reference)Quick Overview for SH3BP1 抗体 (C-Term) (ABIN185183)
抗原
See all SH3BP1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- SH3BP1
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序列
- PQPRPRSLAS ETN
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
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亚型
- IgG
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anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody
SH3BP1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SH3-Domain Binding Protein 1 (SH3BP1) (AA 161-266) antibodySH3BP1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SH3-Domain Binding Protein 1 (SH3BP1) (AA 125-174) antibodySH3BP1 适用: 人, 小鼠, Cow, 豚鼠, 马, 大鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.
Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).
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: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).
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- SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
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别名
- SH3BP1
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背景
- SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
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基因ID
- 23616
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NCBI登录号
- NP_061830
抗原
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