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STK35 抗体 (C-Term)

Verified STK35 适用: 人, 小鼠, Pig WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185178
发货至: 中国
  • 抗原 See all STK35 抗体
    STK35 (Serine/threonine Kinase 35 (STK35))
    抗原表位
    • 15
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 26
    • 17
    • 11
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, Pig
    宿主
    • 41
    山羊
    克隆类型
    • 41
    多克隆
    标记
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This STK35 antibody is un-conjugated
    应用范围
    • 30
    • 15
    • 13
    • 13
    • 9
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    Clik1 / STK35
    序列
    ELETRMDQVT CAA
    交叉反应
    Cow, 犬, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." in: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

  • 抗原
    STK35 (Serine/threonine Kinase 35 (STK35))
    别名
    STK35 (STK35 产品)
    别名
    clik1 antibody, stk35l1 antibody, MGC69346 antibody, im:6905214 antibody, zgc:153287 antibody, MGC115406 antibody, CLIK1 antibody, STK35L1 antibody, 1700054C12Rik antibody, Stk35l1 antibody, serine/threonine kinase 35 antibody, serine/threonine kinase 35 S homeolog antibody, stk35 antibody, stk35.S antibody, STK35 antibody, Stk35 antibody
    背景
    STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
    基因ID
    140901, 67333
    NCBI登录号
    NP_543026
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