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PARD6A 抗体 (C-Term)

This 山羊 多克隆 anti-PARD6A antibody specifically detects PARD6A in ELISA, WB, IHC 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN185152
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for PARD6A 抗体 (C-Term) (ABIN185152)

抗原

See all PARD6A 抗体
PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This PARD6A antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 6
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    C-Term

    原理

    PAR6alpha / PARD6A

    序列

    GSRIRGDGSG FSL

    特异性

    This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 μg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocar

    Western Blot: Approx 40 kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4 kDa according to NP_058644.1) . Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

    别名

    PARD6A

    背景

    PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning d

    基因ID

    50855

    NCBI登录号

    NP_058644, NP_001032358

    途径

    Cell-Cell Junction Organization
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