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Pleckstrin 抗体 (C-Term)

This anti-Pleckstrin antibody is a 山羊 多克隆 antibody detecting Pleckstrin in WB, IHC, ELISA, FACS 和 IF. Suitable for 人.
产品编号 ABIN185127
发货至: 中国

Quick Overview for Pleckstrin 抗体 (C-Term) (ABIN185127)

抗原

See all Pleckstrin (PLEK) 抗体
Pleckstrin (PLEK)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Pleckstrin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 5
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    C-Term

    原理

    Pleckstrin

    序列

    EWIKAIQMAS RTGK

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    C-EWIKAIQMASRTGK

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 40-45 kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1 kDa according to Human NP_002655.2). Recommended concentration: 0.0

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Pleckstrin (PLEK)

    别名

    PLEK

    背景

    PLEK, pleckstrin, P47, p47, FLJ27168

    基因ID

    5341

    NCBI登录号

    NP_002655

    途径

    Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process
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