STXBP1 抗体 (C-Term)
Quick Overview for STXBP1 抗体 (C-Term) (ABIN185107)
抗原
See all STXBP1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- C-Term
-
原理
- MUNC18 / STXBP1 (isoform a)
-
序列
- SRVSFEDQAP TME
-
特异性
- This antibody is expected to recognize isoform a (NP_003156.1).
-
交叉反应
- 人, 小鼠
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
免疫原
- Peptide with sequence C-SRVSFEDQAPTME, from the C Terminus of the protein sequence according to NP_003156.1.
-
亚型
- IgG
-
-
-
-
应用备注
-
Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.
Western Blot: Approx 70-75 kDa band observed in Human Cerebellum lysates (calculated MW of 68.7 kDa according to Human NP_003156.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:16000.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
-
Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138950, (2016) (PubMed).
: "
-
Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138950, (2016) (PubMed).
-
- STXBP1 (Syntaxin Binding Protein 1 (STXBP1))
-
别名
- STXBP1
-
背景
- STXBP1, MUNC18-1, UNC18, hUNC18, syntaxin binding protein 1, Hunc18, rbSec1, syntaxin-binding protein 1, RP11-56D16.3, EIEE4, FLJ37475, RBSEC1
-
基因ID
- 6812, 20910
-
NCBI登录号
- NP_003156
-
途径
- Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
-