PPP1R8 抗体 (C-Term)
Quick Overview for PPP1R8 抗体 (C-Term) (ABIN185050)
抗原
See all PPP1R8 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- NIPP1 / PPP1R8
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序列
- EAWPGKKPTP SLLI
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特异性
- This antibody is expected to recognise all three human isoforms of this protein.
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交叉反应
- Cow, 犬, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EAWPGKKPTPSLLI, from the C Terminus of the protein sequence according to NP_002704.1, NP_054829.2, NP_612568.1.
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亚型
- IgG
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anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (AA 1-209) antibody
PPP1R8 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G11 unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (AA 1-209) antibodyPPP1R8 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (AA 28-127) antibodyPPP1R8 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (AA 21-120) antibodyPPP1R8 适用: 小鼠, 斑马鱼 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (AA 71-120) antibodyPPP1R8 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 猴, Pig, Bat, Hamster, 非洲爪蟾, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) antibodyPPP1R8 适用: 人, 小鼠, 大鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (N-Term) antibodyPPP1R8 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 马, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (Internal Region) antibodyPPP1R8 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) antibodyPPP1R8 适用: Archaeoprepona WB 宿主: 山羊 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) (AA 35-84) antibodyPPP1R8 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 猴, Pig, Bat, Hamster, 非洲爪蟾, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8 μg/mL.
Western Blot: Approx 40 kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5 kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 h
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))
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别名
- PPP1R8
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背景
- PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory
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基因ID
- 5511, 100336
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NCBI登录号
- NP_002704, NP_054829, NP_612568
抗原
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