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FOXA1 抗体 (C-Term)

Cited in 1 publication. This 山羊 多克隆 anti-FOXA1 antibody specifically detects FOXA1 in WB, IF, FACS 和 ELISA. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN185021
发货至: 中国
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Quick Overview for FOXA1 抗体 (C-Term) (ABIN185021)

抗原

See all FOXA1 抗体
FOXA1 (Forkhead Box A1 (FOXA1))

适用

  • 186
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人, 小鼠

宿主

  • 98
  • 89
山羊

克隆类型

  • 132
  • 55
多克隆

标记

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This FOXA1 antibody is un-conjugated

应用范围

  • 129
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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    FOXA1 / HNF3A

    序列

    GVYSRPVLNT S

    交叉反应

    Cow, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55 kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1 kDa according to Human NP_004487.2 and 48.9 kDa according to Mouse NP_032285.2)

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bjerke, Pietrzak, Melhuish, Frierson, Paschal, Wotton: "Prostate cancer induced by loss of Apc is restrained by TGFβ signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92800, (2014) (PubMed).

  • 抗原

    FOXA1 (Forkhead Box A1 (FOXA1))

    别名

    FOXA1

    背景

    FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha

    基因ID

    3169, 15375, 25098

    NCBI登录号

    NP_004487

    途径

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
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