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FOXA1 抗体 (C-Term)

Name: FOXA1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN185021
Availability: OutOfStock

This anti-FOXA1 antibody is a 山羊多克隆 antibody detecting FOXA1 in WB, FACS, IF 和 ELISA. Suitable for 人和小鼠. This Primary Antibody has been cited in 1 publication.

ABIN185021 (5µg/ml) staining of paraffin embedded Human Breast. (FOXA1 抗体 (C-Term))

IHC

ABIN185021 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0. (FOXA1 抗体 (C-Term))

FACS

ABIN185021 (2µg/ml) staining of paraffin embedded Human Prostate. (FOXA1 抗体 (C-Term))

IHC

ABIN185021 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. (FOXA1 抗体 (C-Term))

ABIN185021 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (FOXA1 抗体 (C-Term))

8 images

(1 reference)

产品编号 ABIN185021

发货至: 中国

Quick Overview for FOXA1 抗体 (C-Term) (ABIN185021)

抗原

See all FOXA1 抗体

FOXA1 (Forkhead Box A1 (FOXA1))

适用

人, 小鼠

宿主

山羊

克隆类型

多克隆

标记

This FOXA1 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

质量等级

Verified

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C-Term

原理

FOXA1 / HNF3A

序列

GVYSRPVLNT S

交叉反应

Cow, 人, 小鼠, Pig, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.

亚型

IgG
anti-Forkhead Box A1 (FOXA1) (AA 367-472) antibody
FOXA1 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 2D7 unconjugated

anti-Forkhead Box A1 (FOXA1) (C-Term) antibody
FOXA1 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 367-472) antibody
FOXA1 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B1 unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 372-472) antibody
FOXA1 适用: 人 WB, IHC, FACS, IF, ISt, StM 宿主: 小鼠 Monoclonal FOXA1-1512 unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 372-472) antibody
FOXA1 适用: 人, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal FOXA1-1518 unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 372-472) antibody
FOXA1 适用: 人, 大鼠 IHC, ISt, StM 宿主: 小鼠 Monoclonal FOXA1-1241 unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 367-472) antibody
FOXA1 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 367-472) antibody
FOXA1 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4F6 unconjugated

anti-Forkhead Box A1 (FOXA1) (AA 372-472) antibody
FOXA1 适用: 人, 大鼠 IHC, ISt, StM 宿主: 兔 Monoclonal FOXA1-2230R unconjugated Recombinant Antibody

anti-Forkhead Box A1 (FOXA1) (AA 263-472) antibody
FOXA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55 kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1 kDa according to Human NP_004487.2 and 48.9 kDa according to Mouse NP_032285.2)
Peptide ELISA: antibody detection limit dilution 1:128000.

说明

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Bjerke, Pietrzak, Melhuish, Frierson, Paschal, Wotton: "Prostate cancer induced by loss of Apc is restrained by TGFβ signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92800, (2014) (PubMed).
抗原

FOXA1 (Forkhead Box A1 (FOXA1))

别名

FOXA1

背景

FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha

基因ID

3169, 15375, 25098

NCBI登录号

NP_004487

途径

Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis