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IFIH1 抗体 (N-Term)

This anti-IFIH1 antibody is a 山羊 多克隆 antibody detecting IFIH1 in WB, ELISA, IHC 和 FACS. Suitable for 人 和 猴. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN185002
发货至: 中国

Quick Overview for IFIH1 抗体 (N-Term) (ABIN185002)

抗原

See all IFIH1 抗体
IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

适用

  • 44
  • 14
  • 4
  • 2
  • 2
  • 2
人, 猴

宿主

  • 38
  • 8
  • 1
山羊

克隆类型

  • 36
  • 11
多克隆

标记

  • 41
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  • 2
  • 1
This IFIH1 antibody is un-conjugated

应用范围

  • 37
  • 23
  • 21
  • 12
  • 8
  • 7
  • 7
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    原理

    MDA5 / IFIH1

    序列

    SNGYSTDENF RYL

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence SNGYSTDENFRYL-C, from the N Terminus of the protein sequence according to NP_071451.2.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10 μg/mL.

    Western Blot: This antibody has been successfully used in Western blot on African Green Monkey: Sato S et al, Arthritis Rheum. 2009 Jul,60(7):2193-200. PMID: 19565506.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sato, Hoshino, Satoh, Fujita, Kawakami, Fujita, Kuwana et al.: "RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial ..." in: Arthritis and rheumatism, Vol. 60, Issue 7, pp. 2193-200, (2009) (PubMed).

    Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." in: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, (2008) (PubMed).

    Bao, Sinha, Liu, Hong, Luxon, Garofalo, Casola: "Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis." in: Virology, Vol. 374, Issue 1, pp. 114-27, (2008) (PubMed).

  • 抗原

    IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

    别名

    IFIH1

    背景

    IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1, helicard

    基因ID

    64135

    NCBI登录号

    NP_071451

    途径

    Activation of Innate immune Response
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