NUP50 抗体 (C-Term)
Quick Overview for NUP50 抗体 (C-Term) (ABIN184968)
抗原
See all NUP50 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- C-Term
-
原理
- Npap60 / Nup50
-
序列
- ELHKILLEKK DA
-
特异性
- This antibody is expected to recognise both reported isoforms (NP_009103.2, NP_705931.1).
-
交叉反应
- Cow, 人, 小鼠, 大鼠
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
免疫原
- Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2, NP_705931.1.
-
亚型
- IgG
-
-
-
-
应用备注
-
Immunohistochemistry: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.
Western Blot: Approx 60 kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
-
: "Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).
-
: "Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).
-
- NUP50 (Nucleoporin 50kDa (NUP50))
-
别名
- NUP50
-
背景
- NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50 kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530
-
基因ID
- 10762, 18141
-
NCBI登录号
- NP_009103, NP_705931
-
途径
- Tube Formation
抗原
-