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NUP50 抗体 (C-Term)

Cited in 1 publication. The 山羊 多克隆 anti-NUP50 antibody (ABIN184968) specifically detects NUP50 in WB, IHC 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN184968
发货至: 中国
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Quick Overview for NUP50 抗体 (C-Term) (ABIN184968)

抗原

See all NUP50 抗体
NUP50 (Nucleoporin 50kDa (NUP50))

适用

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宿主

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山羊

克隆类型

  • 62
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多克隆

标记

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This NUP50 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    Npap60 / Nup50

    序列

    ELHKILLEKK DA

    特异性

    This antibody is expected to recognise both reported isoforms (NP_009103.2, NP_705931.1).

    交叉反应

    Cow, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2, NP_705931.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.

    Western Blot: Approx 60 kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Shitashige, Satow, Honda, Ono, Hirohashi, Yamada: "Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).

  • 抗原

    NUP50 (Nucleoporin 50kDa (NUP50))

    别名

    NUP50

    背景

    NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50 kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530

    基因ID

    10762, 18141

    NCBI登录号

    NP_009103, NP_705931

    途径

    Tube Formation
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