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TRIM21 抗体 (C-Term)

This anti-TRIM21 antibody is a 山羊 多克隆 antibody detecting TRIM21 in WB, ELISA, IF 和 FACS. Suitable for 人.
产品编号 ABIN184965
发货至: 中国

Quick Overview for TRIM21 抗体 (C-Term) (ABIN184965)

抗原

See all TRIM21 抗体
TRIM21 (Tripartite Motif Containing 21 (TRIM21))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This TRIM21 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    SSA1 / Ro52

    序列

    CPLNIGSQGS TDY

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence CPLNIGSQGSTDY, from the C Terminus of the protein sequence according to NP_003132.2.

    亚型

    IgG
  • 应用备注

    Western Blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antib

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    TRIM21 (Tripartite Motif Containing 21 (TRIM21))

    别名

    TRIM21

    背景

    TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52 kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome ant

    基因ID

    6737

    NCBI登录号

    NP_003132
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