TRIM55 抗体 (C-Term)
Quick Overview for TRIM55 抗体 (C-Term) (ABIN184953)
抗原
See all TRIM55 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- MURF2 / TRIM55
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序列
- HIFSFSWLNS LNE
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特异性
- This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
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交叉反应
- 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1, NP_908974.1, NP_908975.1.
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亚型
- IgG
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anti-Tripartite Motif Containing 55 (TRIM55) (AA 1-452) antibody
TRIM55 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 101-300) antibodyTRIM55 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 1-452) antibodyTRIM55 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 216-250) antibodyTRIM55 适用: 人 WB 宿主: 兔 Polyclonal RB53569 unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 295-329) antibodyTRIM55 适用: 人 WB 宿主: 兔 Polyclonal RB53681 unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 1-452) antibodyTRIM55 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 72-121) antibodyTRIM55 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, Pig, Bat, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 72-121) antibodyTRIM55 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, Pig, 斑马鱼, Bat, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (AA 183-452) antibodyTRIM55 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 55 (TRIM55) (N-Term) antibodyTRIM55 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.
Western Blot: Approx 58 kDa band observed in lysates of cell line HeLa (calculated MW of 60.5 kDa according to NP_908973.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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