RNF13 抗体 (C-Term)
Quick Overview for RNF13 抗体 (C-Term) (ABIN184935)
抗原
See all RNF13 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- RNF13 / RZF (isoform 1 and 3)
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序列
- PNGERDYNIA NTV
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特异性
- Reported variants represent identical protein (NP_009213.1, NP_899237.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-PNGERDYNIANTV, from the C Terminus of the protein sequence according to NP_009213.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: This antibody stained islets but not in ducts and acini of Human normal pancreas. However, in pancreatic ductal adenocarcinoma staining was in the ducts and acini.
Western Blot: Approx. 42 kDa band observed in lysates of cell line A431 and in preliminary testing of Human Lung lysate, and approx. 45 kDa in lysates of cell line HeLa and in preliminary testing of U251 cell lysate (calculated MW of 42.8 kDa according to
Peptide ELISA: antibody detection limit dilution 1:1000.
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说明
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Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- RNF13 (Ring Finger Protein 13 (RNF13))
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别名
- RNF13
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背景
- RNF13, ring finger protein 13, RZF, RING zinc finger protein, MGC13689, FLJ93817
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基因ID
- 11342
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NCBI登录号
- NP_009213
抗原
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