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ARIH2 抗体 (C-Term)

Cited in 1 publication. This 山羊 多克隆 anti-ARIH2 antibody specifically detects ARIH2 in WB, ELISA 和 IF. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN184916
发货至: 中国
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Quick Overview for ARIH2 抗体 (C-Term) (ABIN184916)

抗原

See all ARIH2 抗体
ARIH2 (protein ariadne-2 homolog (ARIH2))

适用

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人, 小鼠

宿主

  • 39
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山羊

克隆类型

  • 40
  • 3
多克隆

标记

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This ARIH2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    ARIH2 / TRIAD1

    序列

    EQRRRTLLKD FHDT

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 60 kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8 kDa according to NP_006312.1) Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60 kDa in lys

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

  • 抗原

    ARIH2 (protein ariadne-2 homolog (ARIH2))

    别名

    ARIH2

    背景

    ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2

    基因ID

    10425, 23807

    NCBI登录号

    NP_006312
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