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PUF60 抗体 (C-Term)

This 山羊 多克隆 antibody specifically detects PUF60 in WB, IHC 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN184855
发货至: 中国
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Quick Overview for PUF60 抗体 (C-Term) (ABIN184855)

抗原

See all PUF60 抗体
PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

适用

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宿主

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山羊

克隆类型

  • 65
  • 6
多克隆

标记

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This PUF60 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

    • 15
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    C-Term

    原理

    SIAHBP1 / FIR

    序列

    YDQERFDNSD LSA

    特异性

    This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1, NP_055096.2, NP_001129505.1)

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-YDQERFDNSDLSA, from the C Terminus of the protein sequence according to NP_510965.1, NP_055096.2, NP_001129505.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Prostate and Skin. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 60 kDa band observed in Human lysates of HEK293 (calculated MW of 59.9 kDa according to NP_510965.1 and 58.2 according to NP_055096.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

    别名

    PUF60

    背景

    PUF60, SIAHBP1, FIR, fuse-binding protein-interacting repressor, RoBPI, siah binding protein 1, poly-U binding splicing factor PUF60, Ro ribonucleoprotein-binding protein 1, pyrimidine tract binding splicing factor, poly-U binding splicing factor 60KDa, F

    基因ID

    22827, 67959

    NCBI登录号

    NP_510965, NP_055096, NP_001129505
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