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ARL6IP5 抗体 (C-Term)

This anti-ARL6IP5 antibody is a 山羊 多克隆 antibody detecting ARL6IP5 in WB, ELISA, IHC, IF 和 FACS. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN184829
发货至: 中国

Quick Overview for ARL6IP5 抗体 (C-Term) (ABIN184829)

抗原

See all ARL6IP5 (Arl6ip5) 抗体
ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))

适用

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宿主

  • 36
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山羊

克隆类型

  • 37
多克隆

标记

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This ARL6IP5 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 7
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    C-Term

    原理

    GTRAP3-18 / JWA

    序列

    NRLTDYISKV KE

    特异性

    C2 passed G1 as 20 kDa in HBR, C2P2 fail in _enh, try HBAM, HBCR, HBFC BP08/10/2021, 22+48 kDa in HBAM-B104, faint 48 kDa in HBAM-B201, multiple bannd in > 37 kDa in both HBCR, multiple bands inclu 22 kDa in both HBFC, opt in HBAM-B201 at 2 μg/mL , rpt HBAM-B10

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-NRLTDYISKVKE, from the C Terminus of the protein sequence according to NP_006398.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 22 kDa band observed in Human Cerebellumn lysates and in lysates of cell line K562 (calculated MW of 21.6 kDa according to NP_006398.1). An additional band of 75-100 kDa was also observed in some lysates.and was successfully blocked by

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the ER/ plasma membranes and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Wang, Gong, Chen, Liu, Li, Li, Zhou: "JWA regulates XRCC1 and functions as a novel base excision repair protein in oxidative-stress-induced DNA single-strand breaks." in: Nucleic acids research, Vol. 37, Issue 6, pp. 1936-50, (2009) (PubMed).

  • 抗原

    ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))

    别名

    ARL6IP5

    背景

    ARL6IP5, GTRAP3-18, JWA, ADP-ribosylation-like factor 6 interacting protein 5, DERP11, HSPC127, PRAF3, addicsin, hp22, jmx, PRA1 domain family 3, cytoskeleton related vitamin A responsive protein, dermal papilla derived protein 11, glutamate transporter E

    基因ID

    10550

    NCBI登录号

    NP_006398

    途径

    Dicarboxylic Acid Transport
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