ARL6IP5 抗体 (C-Term)
Quick Overview for ARL6IP5 抗体 (C-Term) (ABIN184829)
抗原
See all ARL6IP5 (Arl6ip5) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- GTRAP3-18 / JWA
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序列
- NRLTDYISKV KE
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特异性
- C2 passed G1 as 20 kDa in HBR, C2P2 fail in _enh, try HBAM, HBCR, HBFC BP08/10/2021, 22+48 kDa in HBAM-B104, faint 48 kDa in HBAM-B201, multiple bannd in > 37 kDa in both HBCR, multiple bands inclu 22 kDa in both HBFC, opt in HBAM-B201 at 2 μg/mL , rpt HBAM-B10
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-NRLTDYISKVKE, from the C Terminus of the protein sequence according to NP_006398.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 22 kDa band observed in Human Cerebellumn lysates and in lysates of cell line K562 (calculated MW of 21.6 kDa according to NP_006398.1). An additional band of 75-100 kDa was also observed in some lysates.and was successfully blocked by
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the ER/ plasma membranes and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "JWA regulates XRCC1 and functions as a novel base excision repair protein in oxidative-stress-induced DNA single-strand breaks." in: Nucleic acids research, Vol. 37, Issue 6, pp. 1936-50, (2009) (PubMed).
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: "JWA regulates XRCC1 and functions as a novel base excision repair protein in oxidative-stress-induced DNA single-strand breaks." in: Nucleic acids research, Vol. 37, Issue 6, pp. 1936-50, (2009) (PubMed).
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- ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))
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别名
- ARL6IP5
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背景
- ARL6IP5, GTRAP3-18, JWA, ADP-ribosylation-like factor 6 interacting protein 5, DERP11, HSPC127, PRAF3, addicsin, hp22, jmx, PRA1 domain family 3, cytoskeleton related vitamin A responsive protein, dermal papilla derived protein 11, glutamate transporter E
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基因ID
- 10550
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NCBI登录号
- NP_006398
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途径
- Dicarboxylic Acid Transport
抗原
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