UBE2C 抗体 (C-Term)
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Quick Overview for UBE2C 抗体 (C-Term) (ABIN184805)
抗原
See all UBE2C 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- UBE2C / UBCH10
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序列
- QETYSKQVTS QEP
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特异性
- This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 70-179) antibody
UBE2C 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 9D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F5D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB2550 unconjugated Recombinant Antibody
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (C-Term) antibodyUBE2C 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
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别名
- UBE2C
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背景
- UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1
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基因ID
- 11065
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NCBI登录号
- NP_008950, NP_861515, NP_861516, NP_861517, NP_861518, NP_001268670
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途径
- Ubiquitin Proteasome Pathway
抗原
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