PAX8 抗体 (N-Term)
Quick Overview for PAX8 抗体 (N-Term) (ABIN184783)
抗原
See all PAX8 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- PAX8 (N Terminus)
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序列
- PHNSIRSGHG GLN
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特异性
- This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Kidney Cortex. Recommended concentration: 5 μg/mL.
Western Blot: Approx 35 kDa band observed in Human Kidney lysates. An additional 48 kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9 kDa according to Human NP_039247.1 PAX8D and 48.2 kDa according to Human NP_003457.1 PAXA).
Peptide ELISA: antibody detection limit dilution 1:128000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- PAX8 (Paired Box 8 (PAX8))
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别名
- PAX8
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背景
- PAX8, paired box gene 8, paired domain gene 8, paired box 8
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基因ID
- 7849, 18510, 81819
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NCBI登录号
- NP_003457, NP_039245, NP_039246, NP_039247, NP_054698
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途径
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation
抗原
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