KIF5B 抗体 (C-Term)
(5 references)Quick Overview for KIF5B 抗体 (C-Term) (ABIN184774)
抗原
See all KIF5B 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Kinesin 1 / UKHC
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序列
- QPVAVRGGGG KQV
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
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亚型
- IgG
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anti-Kinesin Family Member 5B (KIF5B) antibody
KIF5B 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (AA 650-770) antibodyKIF5B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (AA 864-962) antibodyKIF5B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A11 unconjugated
anti-Kinesin Family Member 5B (KIF5B) (N-Term) antibodyKIF5B 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (AA 338-514) antibodyKIF5B 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (AA 694-743) antibodyKIF5B 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 马, 兔, 小鸡, 豚鼠, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibodyKIF5B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (N-Term) antibodyKIF5B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibodyKIF5B 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 斑马鱼, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 5B (KIF5B) (C-Term) antibody (Biotin)Verified KIF5B 适用: 人, 犬 WB, IHC, ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in
Peptide ELISA: antibody detection limit dilution 1:8000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).
: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).
: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).
: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).
: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).
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: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).
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- KIF5B (Kinesin Family Member 5B (KIF5B))
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别名
- KIF5B
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背景
- KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
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基因ID
- 3799
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NCBI登录号
- NP_004512
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途径
- Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization
抗原
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