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Flotillin 1 抗体 (C-Term)

Name: Flotillin 1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN184756
Availability: OutOfStock

This anti-Flotillin 1 antibody is a 山羊多克隆 antibody detecting Flotillin 1 in WB, ELISA 和 IHC. Suitable for 人. This Primary Antibody has been cited in 1 publication.

ABIN184756 (0. (Flotillin 1 抗体 (C-Term))

(ABIN184756) Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody. (Flotillin 1 抗体 (C-Term))

IHC

HEK293 overexpressing FLOT1(RC200231) and probed with ABIN184756 (mock transfection in first lane), tested by Origene. (Flotillin 1 抗体 (C-Term))

ABIN184756 (3. (Flotillin 1 抗体 (C-Term))

IHC

ABIN184756 staining (0. (Flotillin 1 抗体 (C-Term))

(ABIN184756) (5 μg/mL) staining of paraffin embedded Human Kidney. (Flotillin 1 抗体 (C-Term))

IHC

6 images

(1 reference)

产品编号 ABIN184756

发货至: 中国

Datasheet as PDF

Quick Overview for Flotillin 1 抗体 (C-Term) (ABIN184756)

抗原

See all Flotillin 1 (FLOT1) 抗体

Flotillin 1 (FLOT1)

适用

人

宿主

山羊

克隆类型

多克隆

标记

This Flotillin 1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified

抗原表位
8

6

5

3

3

2

2

2

2

1

1

1

1

1
C-Term

原理

Flotillin 1

序列

SISQVNHKPL RTA

特异性

This antibody is expected to recognize both reported isoforms (NP_005794.1, NP_001305804.1).

交叉反应

Cow, 犬, 人, Pig

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

C-SISQVNHKPLRTA

亚型

IgG
anti-Flotillin 1 (FLOT1) (AA 169-251) antibody
FLOT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Flotillin 1 (FLOT1) (AA 128-427) antibody
FLOT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Flotillin 1 (FLOT1) (C-Term) antibody
KO Validated FLOT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Flotillin 1 (FLOT1) (AA 101-200) antibody
FLOT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Flotillin 1 (FLOT1) (AA 1-427) antibody
FLOT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Flotillin 1 (FLOT1) (C-Term) antibody (Biotin)
Verified FLOT1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal Biotin

anti-Flotillin 1 (FLOT1) antibody
FLOT1 适用: 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 6C10 unconjugated

anti-Flotillin 1 (FLOT1) (AA 219-234), (Middle Region) antibody
FLOT1 适用: 人, 小鼠, 大鼠 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Flotillin 1 (FLOT1) (AA 104-133), (N-Term) antibody
FLOT1 适用: 人 WB 宿主: 兔 Polyclonal RB40153 unconjugated

anti-Flotillin 1 (FLOT1) antibody
FLOT1 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), FACS 宿主: 兔 Monoclonal 4C3 unconjugated

应用备注

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperatu
Peptide ELISA: antibody detection limit dilution 1:32000.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).
抗原

Flotillin 1 (FLOT1)

别名

FLOT1

背景

FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2

基因ID

10211, 14251

NCBI登录号

NP_005794, NP_001305804