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Flotillin 1 抗体 (C-Term)

Cited in 1 publication. This 山羊 多克隆 anti-Flotillin 1 antibody specifically detects Flotillin 1 in WB, ELISA 和 IHC. The antibody is reactive with 人 samples.
产品编号 ABIN184756
发货至: 中国
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Quick Overview for Flotillin 1 抗体 (C-Term) (ABIN184756)

抗原

See all Flotillin 1 (FLOT1) 抗体
Flotillin 1 (FLOT1)

适用

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宿主

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山羊

克隆类型

  • 43
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多克隆

标记

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This Flotillin 1 antibody is un-conjugated

应用范围

  • 48
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    Flotillin 1

    序列

    SISQVNHKPL RTA

    特异性

    This antibody is expected to recognize both reported isoforms (NP_005794.1, NP_001305804.1).

    交叉反应

    Cow, 犬, 人, Pig

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    C-SISQVNHKPLRTA

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4 kDa according to NP_005794.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperatu

    Peptide ELISA: antibody detection limit dilution 1:32000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).

  • 抗原

    Flotillin 1 (FLOT1)

    别名

    FLOT1

    背景

    FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2

    基因ID

    10211, 14251

    NCBI登录号

    NP_005794, NP_001305804
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