Melanophilin 抗体 (C-Term)
(4 references)Quick Overview for Melanophilin 抗体 (C-Term) (ABIN184753)
抗原
See all Melanophilin (MLPH) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Melanophilin (mouse)
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序列
- ARHIFAKPVM AQQP
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交叉反应
- 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-ARHIFAKPVMAQQP, from the C Terminus of the protein sequence according to NP_443748.2.
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亚型
- IgG
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anti-Melanophilin (MLPH) (AA 310-539) antibody
MLPH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (C-Term) antibodyMLPH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 301-600) antibodyMLPH 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 511-560) antibodyMLPH 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 1-600) antibodyMLPH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 541-569), (C-Term) antibodyMLPH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32075 unconjugated
anti-Melanophilin (MLPH) (AA 540-569), (C-Term) antibodyMLPH 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/mL. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22,283(34):23209-16. PMID:18559336.
GST-pull do -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).
: "Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion." in: The FEBS journal, Vol. 280, Issue 3, pp. 892-903, (2013) (PubMed).
: "Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23209-16, (2008) (PubMed).
: "A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes." in: Molecular biology of the cell, Vol. 17, Issue 11, pp. 4720-35, (2006) (PubMed).
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: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).
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- Melanophilin (MLPH)
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别名
- Mlph
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背景
- 2210418F23Rik, melanophilin, Mlph, ln, l1Rk3, Slac-2a, D1Wsu84e, l(1)-3Rk, 5031433I09Rik, leaden, AW228792, OTTMUSP00000025451
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基因ID
- 171531, 316620
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NCBI登录号
- NP_443748
抗原
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