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DAP3 抗体 (C-Term)

This anti-DAP3 antibody is a 山羊 多克隆 antibody detecting DAP3 in WB, ELISA, IHC 和 IF. Suitable for 人.
产品编号 ABIN184743
发货至: 中国

Quick Overview for DAP3 抗体 (C-Term) (ABIN184743)

抗原

See all DAP3 抗体
DAP3 (Death-Associated Protein 3 (DAP3))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This DAP3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    DAP3

    序列

    NPSLLERHCA YL

    特异性

    This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1, NP_001186779.1, NP_001186780.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 40 kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41 kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1 μg/mL. Primary incubated 1 hour at room

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    DAP3 (Death-Associated Protein 3 (DAP3))

    别名

    DAP3

    背景

    DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29

    基因ID

    7818

    NCBI登录号

    NP_387506, NP_001186779, NP_001186780
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