SIAH1 抗体 (N-Term)
(1 reference)Quick Overview for SIAH1 抗体 (N-Term) (ABIN184675)
抗原
See all SIAH1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- SIAH1
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序列
- SRQTATALPT GTSKC
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特异性
- This product is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1)
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.
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亚型
- IgG
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anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibody
SIAH1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibodySIAH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 191-240) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔, 非洲爪蟾, 小鸡, Bat, Hamster, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (C-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 201-282) antibodySIAH1 适用: 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibodySIAH1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody (Biotin)Verified SIAH1 适用: 人, 大鼠, 小鼠 WB, ELISA, IP 宿主: 山羊 Polyclonal Biotin
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibodySIAH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F26 unconjugated
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应用备注
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Western Blot: Approx. 37 kDa band observed in Human Liver lysates and approx. 30+37 kDa bands in Rat Liver lysates (calculated MW of 34.6 kDa according to Human NP_001006611.1 and 31.1 kDa according to Rat NP_543181.2 and 38.2 kDa according to Rat XP_03895336
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).
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: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).
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- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
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别名
- SIAH1
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背景
- SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog
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分子量
- 31.1kDa
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基因ID
- 6477, 20437, 140941
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NCBI登录号
- NP_003022, NP_001006611
抗原
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