STAP2 抗体 (C-Term)
Quick Overview for STAP2 抗体 (C-Term) (ABIN184610)
抗原
See all STAP2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- STAP2 / BKS
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序列
- ELQKKLEKRR ALEH
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特异性
- This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-ELQKKLEKRRALEH, from the C Terminus of the protein sequence according to NP_060190.2, NP_001013863.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 48 kDa band observed in Human Heart lysates and 45 kDa band observed in Human Breast Lysates (calculated MW of 49.6 kDa according to NP_060190 and of NP_001013863.1). Recommended concentration: 0.5-1.5 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells." in: Cancer science, Vol. 102, Issue 4, pp. 756-61, (2011) (PubMed).
: "Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 49, pp. 38093-103, (2010) (PubMed).
: "STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation." in: Biochemical and biophysical research communications, Vol. 384, Issue 1, pp. 71-5, (2009) (PubMed).
: "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7966-74, (2009) (PubMed).
: "
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Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells." in: Cancer science, Vol. 102, Issue 4, pp. 756-61, (2011) (PubMed).
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- STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))
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别名
- STAP2
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背景
- STAP2, BKS, STAP-2, FLJ20234, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein-2, signal transducing adaptor family member 2
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基因ID
- 55620
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NCBI登录号
- NP_060190, NP_001013863
抗原
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