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STAP2 抗体 (C-Term)

This anti-STAP2 antibody is a 山羊 多克隆 antibody detecting STAP2 in WB 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN184610
发货至: 中国

Quick Overview for STAP2 抗体 (C-Term) (ABIN184610)

抗原

See all STAP2 抗体
STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

适用

  • 34
  • 2
  • 1

宿主

  • 32
  • 2
  • 1
山羊

克隆类型

  • 34
  • 1
多克隆

标记

  • 16
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This STAP2 antibody is un-conjugated

应用范围

  • 35
  • 15
  • 13
  • 13
  • 11
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA

质量等级

Verified
  • 抗原表位

    • 15
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    STAP2 / BKS

    序列

    ELQKKLEKRR ALEH

    特异性

    This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-ELQKKLEKRRALEH, from the C Terminus of the protein sequence according to NP_060190.2, NP_001013863.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 48 kDa band observed in Human Heart lysates and 45 kDa band observed in Human Breast Lysates (calculated MW of 49.6 kDa according to NP_060190 and of NP_001013863.1). Recommended concentration: 0.5-1.5 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Ikeda, Mizushima, Sekine, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells." in: Cancer science, Vol. 102, Issue 4, pp. 756-61, (2011) (PubMed).

    Ikeda, Sekine, Mizushima, Nakasuji, Miyasaka, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 49, pp. 38093-103, (2010) (PubMed).

    Ikeda, Miyasaka, Sekine, Mizushima, Muromoto, Nanbo, Yoshimura, Matsuda: "STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation." in: Biochemical and biophysical research communications, Vol. 384, Issue 1, pp. 71-5, (2009) (PubMed).

    Sekine, Ikeda, Tsuji, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7966-74, (2009) (PubMed).

  • 抗原

    STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

    别名

    STAP2

    背景

    STAP2, BKS, STAP-2, FLJ20234, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein-2, signal transducing adaptor family member 2

    基因ID

    55620

    NCBI登录号

    NP_060190, NP_001013863
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